Cell Culture:Article Title: Mutant p53 drives cancer chemotherapy resistance due to loss of function on activating transcription of PUMA
Article Snippet: .. Cell culture and treatment The human CRC cell lines expressing wild-type or mutant p53 (HCT-116, SW620, SW480), plus Pancreatic cancer cell (PANC-1) and Breast cancer cell (SKBR-3) were obtained from American type culture collection (ATCC). .. SW620 and SW480 were cultured in L-15 (KeyGEN BioTECH) modified media and HCT116 were routinely cultured in McCoy’s 5A (gibicol) modified media and other cell lines were cultured in DMEM (gibicol), supplemented with 10% fetal bovine serum (FBS), penicillin (100 units/mL), and streptomycin (100 mg/mL) in 5% CO2 at 37°C in humidified incubator.
Article Title: CCDC106 promotes the proliferation and invasion of ovarian cancer cells by suppressing p21 transcription through a p53-independent pathway
Article Snippet: .. Mutant p53 (SKOV3, OVCAR3) and wild-type p53 (A2780) ovarian cancer cell lines were obtained from the ATCC (Manassas, VA, USA) and were cultured in RPMI-1640 (Invitrogen, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum FBS (Invitrogen, Carlsbad, CA, USA), 100 IU/ml penicillin (Sigma, St. Louis, MO, USA) and 100 μg/ml streptomycin (Sigma, St. Louis, MO, USA) in 5% CO 2 at 37°C. .. The mutant p53 (CAOV3) ovarian cancer cell line was obtained from the Shanghai Cell Bank (Shanghai, China) and was cultured in DMEM (Invitrogen, Carlsbad, CA, USA).
Article Title: CCDC106 promotes the proliferation and invasion of ovarian cancer cells by suppressing p21 transcription through a p53-independent pathway.
Article Snippet: .. Mutant p53 (SKOV3, OVCAR3) and wild-type p53 (A2780) ovarian cancer cell lines were obtained from the ATCC (Manassas, VA, USA) and were cultured in RPMI-1640 (Invitrogen, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum FBS (Invitrogen, Carlsbad, CA, USA), 100 IU/ml penicillin (Sigma, St. Louis, MO, USA) and 100 μg/ml streptomycin (Sigma, St. Louis, MO, USA) in 5% CO2 at 37°C. .. The mutant p53 (CAOV3) ovarian cancer cell line was obtained from the Shanghai Cell Bank (Shanghai, China) and was cultured in DMEM (Invitrogen, Carlsbad, CA, USA).
Expressing:Article Title: Mutant p53 drives cancer chemotherapy resistance due to loss of function on activating transcription of PUMA
Article Snippet: .. Cell culture and treatment The human CRC cell lines expressing wild-type or mutant p53 (HCT-116, SW620, SW480), plus Pancreatic cancer cell (PANC-1) and Breast cancer cell (SKBR-3) were obtained from American type culture collection (ATCC). .. SW620 and SW480 were cultured in L-15 (KeyGEN BioTECH) modified media and HCT116 were routinely cultured in McCoy’s 5A (gibicol) modified media and other cell lines were cultured in DMEM (gibicol), supplemented with 10% fetal bovine serum (FBS), penicillin (100 units/mL), and streptomycin (100 mg/mL) in 5% CO2 at 37°C in humidified incubator.
Article Title: Mutant p53 drives cancer chemotherapy resistance due to loss of function on activating transcription of PUMA
Article Snippet: .. The human CRC cell lines expressing wild-type or mutant p53 (HCT-116, SW620, SW480), plus Pancreatic cancer cell (PANC-1) and Breast cancer cell (SKBR-3) were obtained from American type culture collection (ATCC). .. SW620 and SW480 were cultured in L-15 (KeyGEN BioTECH) modified media and HCT116 were routinely cultured in McCoy’s 5A (gibicol) modified media and other cell lines were cultured in DMEM (gibicol), supplemented with 10% fetal bovine serum (FBS), penicillin (100 units/mL), and streptomycin (100 mg/mL) in 5% CO2 at 37°C in humidified incubator.
Mutagenesis:Article Title: Mutant p53 drives cancer chemotherapy resistance due to loss of function on activating transcription of PUMA
Article Snippet: .. Cell culture and treatment The human CRC cell lines expressing wild-type or mutant p53 (HCT-116, SW620, SW480), plus Pancreatic cancer cell (PANC-1) and Breast cancer cell (SKBR-3) were obtained from American type culture collection (ATCC). .. SW620 and SW480 were cultured in L-15 (KeyGEN BioTECH) modified media and HCT116 were routinely cultured in McCoy’s 5A (gibicol) modified media and other cell lines were cultured in DMEM (gibicol), supplemented with 10% fetal bovine serum (FBS), penicillin (100 units/mL), and streptomycin (100 mg/mL) in 5% CO2 at 37°C in humidified incubator.
Article Title: CCDC106 promotes the proliferation and invasion of ovarian cancer cells by suppressing p21 transcription through a p53-independent pathway
Article Snippet: .. Mutant p53 (SKOV3, OVCAR3) and wild-type p53 (A2780) ovarian cancer cell lines were obtained from the ATCC (Manassas, VA, USA) and were cultured in RPMI-1640 (Invitrogen, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum FBS (Invitrogen, Carlsbad, CA, USA), 100 IU/ml penicillin (Sigma, St. Louis, MO, USA) and 100 μg/ml streptomycin (Sigma, St. Louis, MO, USA) in 5% CO 2 at 37°C. .. The mutant p53 (CAOV3) ovarian cancer cell line was obtained from the Shanghai Cell Bank (Shanghai, China) and was cultured in DMEM (Invitrogen, Carlsbad, CA, USA).
Article Title: Protein detection in blood with single-molecule imaging
Article Snippet: LnCaP human prostate cancer cells were obtained from American Type Culture Collection (ATCC). .. Human cells without p53 (BHK21) and with mutant p53 [CFPAC-1 (C242R), OVCAR3 (R248Q), and TOV-112D (R175H)]; cells with wild-type p53 (MCF-7 and MCF-10); and human embryonic kidney 293T cells were from ATCC. .. Cells were cultured in RPMI 1640 medium or Dulbecco’s modified Eagle’s medium (Thermo Fisher Scientific) with 10% fetal bovine serum in the absence of phenol red and maintained under 5% CO 2 atmosphere.
Article Title: Mutant p53 drives cancer chemotherapy resistance due to loss of function on activating transcription of PUMA
Article Snippet: .. The human CRC cell lines expressing wild-type or mutant p53 (HCT-116, SW620, SW480), plus Pancreatic cancer cell (PANC-1) and Breast cancer cell (SKBR-3) were obtained from American type culture collection (ATCC). .. SW620 and SW480 were cultured in L-15 (KeyGEN BioTECH) modified media and HCT116 were routinely cultured in McCoy’s 5A (gibicol) modified media and other cell lines were cultured in DMEM (gibicol), supplemented with 10% fetal bovine serum (FBS), penicillin (100 units/mL), and streptomycin (100 mg/mL) in 5% CO2 at 37°C in humidified incubator.
Article Title: CCDC106 promotes the proliferation and invasion of ovarian cancer cells by suppressing p21 transcription through a p53-independent pathway.
Article Snippet: .. Mutant p53 (SKOV3, OVCAR3) and wild-type p53 (A2780) ovarian cancer cell lines were obtained from the ATCC (Manassas, VA, USA) and were cultured in RPMI-1640 (Invitrogen, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum FBS (Invitrogen, Carlsbad, CA, USA), 100 IU/ml penicillin (Sigma, St. Louis, MO, USA) and 100 μg/ml streptomycin (Sigma, St. Louis, MO, USA) in 5% CO2 at 37°C. .. The mutant p53 (CAOV3) ovarian cancer cell line was obtained from the Shanghai Cell Bank (Shanghai, China) and was cultured in DMEM (Invitrogen, Carlsbad, CA, USA).
Article Title: Methods and materials for treating cancer
Article Snippet: LnCaP human prostate cancer cells were obtained from ATCC. .. Human cells without p53 (BHK21) and with mutant p53 (CFPAC-1 (C242R), OVCAR3 (R248Q), and TOV-112D (R175H)), cells with wildtype p53 (MCF-7, MCF-10), and HEK 293T cells were from ATCC. .. Cells were cultured in RPMI-1640 medium or DMEM (Thermo Fisher Scientific) with 10% FBS in the absence of phenol red and maintained under 5% CO2 atmosphere.
Article Title: Role of Thiol Reactivity for Targeting Mutant p53.
Article Snippet: .. This left two leukemia lines with WT p53 (MOLT-4, SR) and two with mutant p53 (RPMI-8226 E285K and CCRF-CEM R248Q), five melanoma lines with WT p53 (MALME-3M, SKMEL-5, UACC-62, LOX IMVI, and UACC-257) and five with mutant p53 (M14 G266E, SK-MEL-2 G245S, SK-MEL-28 L145R, MDA MB-435 G266E, and MDA-N G266E), two NSCLC lines with WT p53 (A549/ATCC and NCI-H460) and two with mutant p53 (HOP-92 R175L and NCI-H23 M246I), two ovarian cancer lines with WT p53 (IGROV1, OVCAR-4) and one with mutant p53 (OVCAR-3 R248Q), four renal cancer lines with WT p53 (A498, ACHN, CAKI-1, and UO-31) and two with mutant p53 (786-0 P278A and TK-10 L264R). ..
Multiple Displacement Amplification:Article Title: Role of Thiol Reactivity for Targeting Mutant p53.
Article Snippet: .. This left two leukemia lines with WT p53 (MOLT-4, SR) and two with mutant p53 (RPMI-8226 E285K and CCRF-CEM R248Q), five melanoma lines with WT p53 (MALME-3M, SKMEL-5, UACC-62, LOX IMVI, and UACC-257) and five with mutant p53 (M14 G266E, SK-MEL-2 G245S, SK-MEL-28 L145R, MDA MB-435 G266E, and MDA-N G266E), two NSCLC lines with WT p53 (A549/ATCC and NCI-H460) and two with mutant p53 (HOP-92 R175L and NCI-H23 M246I), two ovarian cancer lines with WT p53 (IGROV1, OVCAR-4) and one with mutant p53 (OVCAR-3 R248Q), four renal cancer lines with WT p53 (A498, ACHN, CAKI-1, and UO-31) and two with mutant p53 (786-0 P278A and TK-10 L264R). ..
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